
姓名:刘光磊
学历/职称/职务:博士/副教授
联系方式:
邮箱: liugl@ouc.edu.cn
地址:山东省青岛市市南区鱼山路5号中国海洋大学科学馆313
科研领域描述:海洋微生物多糖水解酶的结构功能研究及酶工程改造;海洋酵母菌天然产物的代谢途径和调控机制研究及代谢工程改造。
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学习工作简历:
2013年7月—2014年11月: 中国海洋大学 海洋生命学院 讲师
2012年7月—2013年6月:中国科学院青岛生物能源与过程研究所 助理研究员
2008年9月—2012年6月:中国海洋大学 海洋生命学院 微生物学专业 博士
2004年9月—2008年6月:中国海洋大学 海洋生命学院 生物科学专业 学士
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代表性成果:
一、承担和参与的课题:
1) 主持,κ-卡拉胶酶中碳水化合物结合模块在酶催化中的作用及其底物识别的分子机制,国家自然科学基金面上项目,25万,2018.01-2019.12
2) 主持,微生物菊粉酶C末端结构域的功能研究,国家自然科学基金青年基金项目,24万,2015.01-2017.12
3) 主持,高效利用菊芋发酵产生物乙醇的酵母工程菌株构建的研究,中国博士后科学基金面上一等资助,8万,2014.9-2015.12
4) 参与,海洋酵母菌菊糖酶基因的高效表达和菊糖酶的中试生产,240万,2013.1-2016.12
5) 参与,以功能基因组学分析大陆和台湾红树林中Aureobasidium和Candida等属的遗传多样性,国家自然科学基金两岸合作项目,200万, 2016.1-2019.12
二、发表论文:
1) Zhao Y#, ChiZ#, Xu Y, Shi N, Chi Z, & LiuGL*. High-levelextracellular expression of κ-carrageenase in Brevibacillus choshinensis forthe production of a series of κ-carrageenan oligosaccharides. ProcessBiochemistry, 2018, 64, 83-92.
2) Han YZ, Zhou CC., Xu,YY., Yao JX., Chi, Z, Chi ZM., & LiuGL*. High-efficient production of fructo-oligosaccharides frominulin by a two-stage bioprocess using an engineered Yarrowia lipolytica strain. Carbohydrate Polymers, 2017, 173, 592.
3) Shi N, Mao W, He X, ChiZ, Chi Z, & Liu G*. (2017). Co-expression of Exo-inulinaseand Endo-inulinase Genes in the Oleaginous Yeast Yarrowia lipolytica forEfficient Single Cell Oil Production from Inulin. Applied biochemistry andbiotechnology, 1-13.
4) Chi Z#, Liu GL#, Liu CG, Chi ZM. Poly (β-l-malic acid)(PMLA)from Aureobasidium spp. and its current proceedings. Appliedmicrobiology and biotechnology, 2016, 100(9): 3841-3851.
5) Liu Y, Zhou S H, ChenYR, Chi Z, Chi ZM, Liu GL*. Synergisticeffect between the recombinant exo-inulinase and endo-inulinase on inulinhydrolysis. Journal of Molecular Catalysis B: Enzymatic,2016, 128: 27-38.
6) Zhou SH, Liu Y, ZhaoYJ, Chi Z, Chi ZM, LiuGL*. Enhanced exo-inulinase activity and stabilityby fusion of an inulin-binding module. Applied microbiology andbiotechnology, 2016, 100(18): 8063–8074 3.376
7) Liu GL,Chi Z, Wang GY, Wang ZP, Li Y, Chi ZM*. Yeastkiller toxins, molecular mechanisms of their action and their applications.Crit Rev Biotechnol. 2015, 35(2)222-234
8) LiuGL, Fu GY, Chi Z, Chi ZM*.Enhanced expression of the codon-optimized exo-inulinase gene from the yeast Meyerozyma guilliermondii in Saccharomyces sp. W0 and bioethanolproduction from inulin. Appl Microbiol Biotechnol. 2014, 98(21): 9129-9138
9) Liu GL#,Chi Z#, Chi ZM*. Molecular characterization andexpression of microbial inulinase genes. Critical reviews in microbiology.2013. 39(2):152-165
10) LiuGL, Li Y, Zhou HX, Chi ZM*,Madzak C. Over-expression of a bacterial chitosanase gene in Yarrowia lipolytica and chitosanhydrolysis by the recombinant chitosanase. J Mol Catal B-Enzym. 2012.83:100-107
11) LiuGL, Wang K, Hua MX, Buzdar MA, Chi ZM*.Purification and characterization of the cold-active killer toxin from thepsychrotolerant yeast Mrakia frigida isolated from sea sediments in Antarctica. Process Biochemistry. 2012.47(5):822-827
12) LiuGL, Wang DS, Wang LF, Zhao SF, Chi ZM*.Mig1 is involved in mycelial formation and expression of the genes encodingextracellular enzymes in Saccharomycopsisfibuligera A11. Fungal Genetics and Biology. 2011. 48(9):904-913
13) LiuGL, Li Y, Chi Z, Chi ZM*.Purification and characterization of kappa-carrageenase from the marinebacterium Pseudoalteromonas porphyrae for hydrolysis of kappa-carrageenan. Process Biochemistry. 2011. 46(1):265-271
Curriculum Vitae
Name: Guanglei Liu
Title: Dr &Associate professor
Contact:
Email:liugl@ouc.edu.cn
Address:College of Marine Life Science, Ocean University ofChina, No. 5 Yushan Road, Qingdao, P.R.China,
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Experiences:
2014.12- , Ocean University of China,Associate professor
2013.07-2014.11, Ocean University of China, Lecturer
2012.7-2013.6, Qingdao Institute ofBioenergy and Bioprocess Technology, Chinese Academy of Sciences, Researchassistant
2008.09-2012.06, OceanUniversity of China, Microbiology, D.Sc
2004.09-2008.06, Ocean University ofChina, Biology, B.Sc.